Abstract
Satellite DNA (satDNA) was purified by repeated neutral CsCl, ethidium bromide/CsCl and actinomycin D/CsCl density centrifugation from total nuclear DNA of Tropeolum majus. Digestins patterns of satDNA and main band DNA as generated with 22 different restriction enzymes were partially characterized and hybridized by the Southern blot technique with Hpa II-generated satDNA fragments and a ribosomal DNA fragment. Some restriction fragments were sequenced by the chemical method, either directly, or after cloning, and possible secondary structures were determined
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