Abstract
The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
Highlights
The differences between OSVII and OSVIII fractions are presented based on MALDI-TOF MS and ESI MSn analysis
The chemical structure and genomics of the complete undecasaccharide core structure of E. tarda EIB 202 LPS are presented for the first time
The functions of the genes found in the waa gene cluster from the E. tarda strain EIB 202 seems to be in agreement with the chemical structure of the LPS-core
Summary
Edwardsiella tarda is a Gram-negative bacterium and a pathogen of farmed fish. It is the etiological agent of a syEstdewmaardtisciedlliaseatasredcaalilsedaeGdwraamrd-nsieegllaotsiivse, wbhaicctherhiuams beaenndreapopratethdotgoeanffeocft afawrmideedrafnisghe. It consists of three moietiews:wlwip.middpAi.c,omco/jroeurnal/ijms oligosaccharide, and O-specific polysaccharide (O-antigen). The O-specific polysaccharide chains are transferred to lipid A-core to form LPS, in a step involving WaaL, the putative bifunctional enzyme named O-antigen ligase. Another interesting feature is the high chemical variability shown by the O-antigen LPS, leading to a similar genetic variation in the genes involved in their biosynthesis, the so-called wb cluster (for a review, see [7]).
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