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Abstract
We present the complete nucleotide sequence of the major beta globin gene of Xenopus laevis with 127 nucleotides of sequence upstream of the gene and 212 nucleotides downstream. The start point of transcription was determined by S1 mapping and primer extension and the site of polyadenylation by comparison with the mRNA sequence determined from a cDNA clone. Comparison of the X. laevis sequence with other genes transcribed by RNA polymerase II reveals conserved features in the 5' flanking, 5' noncoding, intron/exon boundary, and 3' noncoding regions.
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