Abstract
The complete nucleotide sequence (5793 bp) of the cosmid vector pTL5 and the origin of its genetic components has been determined. Cosmid pTL5, a derivative of cosmid vector pHC79, is composed of genetic components from pBR322, bacteriophage λ and the hybrid lambdoid bacteriophage Charon (Ch) 4A cohesive ends ( cos) region. The Ch4A cos region contains genetic components from two bacteriophages, the λ cos-left arm and the φ80 cos-right arm regions. The Ch4A cos region has been used in the construction of many other cosmid-type vectors, some of which have been sequenced and entered into the GenBank database.
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