The complete mitogenome and phylogeny analysis of Pareuchiloglanis longicauda (Yue, 981) (Siluriformes: Sisoridae)

Abstract

Pareuchiloglanis longicauda, a Sisorid fish that is distributed in the upper Pearl River. In this study, the complete mitogenome of P. longicauda was sequenced using traditional Sanger sequencing approach. The 16,588 bp genome was consisted of 2 rRNAs, 22tRNAs, 13 protein-coding genes (PCGs) and 1 control region. The 13 PCGs started with a traditional ATG and end with stop codon TAA, TAG, TGA, TA or a single T base. Phylogenetic analysis based on 13 PCGs from 22 species using maximum-likelihood method produced three major clades (Clade I, II and III). Unexpectedly, our mitogenome exhibited only 92.12% identity to the previously published one (GenBank accession no. KP872693) with differences mainly located in the gene region. Furthermore, Pareuchiloglanis did not form a monophyletic genus and P. longicauda had the closest relationship with P. macrotrema. The result suggested that more complete mitogenomes are needed to reveal the phylogenetic placement of Pareuchiloglanis in the family Sisoridae.