The complete mitochondrial genome sequence of Dodona eugenes (Lepidoptera: Riodinidae)

Zhong-Xue Wei,Gang Sun,Jer-Yuan Shiu,Yan Fang,Qing-Hui Shi

doi:10.1080/23802359.2021.1884014

Abstract

The complete mitochondrial genome (mitogenome) sequence of Dodona eugenes (Lepidoptera: Riodinidae) was determined and analyzed. The mitogenome is 15,680 bp in length with consisting of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes (rrnL and rrnS), and one AT-rich region. The gene content, orientation, and order are identical to that of the majority of other lepidopteran insects. The D. eugenes mitogenome includes a cox1 gene with an atypical CGA(R) start codon and three genes (cox1, nad5, and nad4) exhibiting incomplete stop codons. All tRNAs have a typical secondary cloverleaf structure, except for trnS1 which lacks the dihydrouridine (DHU) arm. The 825-bp long AT-rich region is the longest among sequenced riodinids, which range from 349 to 423 bp. The conclusion of phylogenetic analysis highly supported the monophyly of Riodinidae, which is standing as the sister of the family Lycaenidae.

Highlights

The insect mitochondrial genomes provide effective data for studies on systematic, population genetics, and evolutionary biology (Cameron 2014; Yang et al 2020)
The concatenated protein-coding genes (PCGs) are 11,208 bp long accounting for approximately 71.5% of the mitogenome
All PCGs are initiated by typical ATN, with the exception of cox1 which uses the unusual CGA(R) as observed in most other sequenced butterflies (Kim and Kim 2014; Wu et al 2014)

Summary

Introduction

The insect mitochondrial genomes (mitogenomes) provide effective data for studies on systematic, population genetics, and evolutionary biology (Cameron 2014; Yang et al 2020). For better understanding of the phylogenetic position and higher systematics of riodinids, the complete mitogenome of Dodona eugenes needs to be determined. The specimen was collected from Sanming in Fujian Province, China (coordinates: E117620, N26270), and kept in the laboratory at À20 C under the accession number SQH-20170628.

Results

Conclusion