Abstract
The dusky shark Carcharhinus obscurus is economically important but vulnerable to overharvesting. The complete C. obscurus mitogenome was assembled from approximately 1 million whole genome shotgun sequences using a combination of reference mapping and de novo assembly (mean coverage 59x). This resulted in a 16,706 bp double-stranded circular mitochondrial sequence. Following the consensus vertebrate mtDNA genome, it comprises 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs and has 2 non-coding areas. The A+T (56.9%) versus G+C (43.1%) composition confirmed an A+T bias previously noted for sharks. This genome is the first for the speciose Carcharhinus genus and provides a valuable resource for studies of shark molecular systematics, phylogeography, conservation genetics, and stock structure.
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