Abstract
In this study, we report the complete mitochondrial genome sequence of the Endangered Band-rumped Storm Petrel (Oceanodroma castro), a globally distributed seabird. The mitogenome is 17,023 bp in length and has a base composition of A (30.5%), T (24.0%), C (31.2%), and G (14.3%). Similar to other avifauna, it contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region, with arrangement and orientation identical to that of other seabirds. To our knowledge, this is the first complete mitochondrial genome sequenced within the family Hydrobatidae, or storm petrels, and will aid in taxonomic studies.
Highlights
The Band-rumped Storm Petrel (Oceanodroma castro), globally distributed, was recently listed under the Endangered Species Act in the Pacific region (USFWS 2015)
Due to low DNA yield, whole genome amplification was performed on individual samples with the REPLI-g UltraFast Mini-kit (Qiagen, Valencia, CA)
The pooled libraries were digested with the frequent-cutter restriction enzyme DpnII from New England BiolabsVR (Ipswich, MA), and fragments between 300 and 700 bp in length were prepared for sequencing on the IlluminaVR MiSeq using the Kapa Biosystems (Wilmington, MA) Hyper Prep kit
Summary
KEYWORDS Endangered species; seabird; mitogenome; Procellariiformes; RADseq The Band-rumped Storm Petrel (Oceanodroma castro), globally distributed, was recently listed under the Endangered Species Act in the Pacific region (USFWS 2015). We sequenced the complete mitochondrial genome of O. castro (GenBank accession number MK170187).
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