Abstract
We sequenced the mitochondrial genome of Pseudorasbora interrupta using Illumina technology and additional Sanger sequencing. The assembled 16601 bp mitogenome had a GC content of 40.98% and consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one non-coding control region (CR), with a gene order identical to the fishes. In addition, we downloaded the mitogenome of the closely related species Topmouth Gudgeon P. parva. The mitogenomes of P. interrupta and P. parva showed a sequence identity of 99.2% with the previously published P. parva mitogenome. The phylogenetic reconstruction based on 14 Gobioninae mitogenomes supported P. interrupta and its sister species P. parva as a monophyletic group. However, Pseudorasbora was proved to be a polyphyletic group which means amendments will be needed for the taxonomy of this genus.
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