Abstract
The fathead minnow, Pimephales promelas, is an ecological important fish distributed across North America. In this study, we firstly assembled the complete mitochondrial genome of P. promelas. The mitogenome is 16 709 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions. The gene content and order is in accord with the common vertebrate form. ATG acts as the start codon for all PCGs except cytochrome c oxidase subunit I (COXI), which has GTG as a start codon. The nucleotide base composition of H-strand is 29.2% A, 27.9% T, 25.9% C, and 17.0% G. The complete mitochondrial genome data would be useful for further studies of reproduction, development, and adaptive evolution of P. promelas.
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