The complete mitochondrial genome of parasitoid wasp Pseudoligosita yasumatsui (Hymenoptera: Trichogrammatidae) and its phylogenetic implications

Abstract

Pseudoligosita yasumatsui (Viggiani and Rao, 1978) (Hymenoptera: Trichogrammatidae) is an egg parasitoid wasp that preys on rice planthoppers, one of the most significant pests in Asian rice-growing regions. An enriched mitochondrial genome database of rice planthopper egg parasitoids is the basis for phylogenetic analysis. Therefore, the complete mitochondrial genome of P. yasumatsui was sequenced using NGS sequencing. The total length of the mitochondrial genome of P. yasumatsui is 16,036 bp, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a control region (CR). The mitochondrial genome showed a negative AT skew (-0.09) and a positive GC skew (0.19). Thirteen PCGs all started with ATN codons and ended with TAA. All 22 tRNAs have a typical cloverleaf structure. A comparison with the presumed arthropod ancestral mitochondrial genome using CREx web serve observed a large number of tRNAs as well as PCGs inversions, rearrangements, and inversion reversals of large gene segments (trnE-rrnL). Based on the Bayesian inference (BI) and maximum likelihood (ML) phylogenetic tree of P. yasumatsui and other 14 mitochondrial genomes from the Chalcidoidea superfamily, the phylogenetic relationship of P. yasumatsui within the Trichogrammatidae family was found to be ((Pseudoligosita + Megaphragma) + Trichogamma), and the phylogenetic relationship of five families was (Mymaridae + (Trichogrammatidae + (Aphelinidae + (Eulophidae + Pteromalidae)))). The close relationship between Pseudoligosita and Megaphragma as sister groups was suggested, and the data provide support for the monophyly of Oligositini.