Abstract
The mitogenome of Neoheterobothrium hirame from olive flounder (Paralichthys olivaceus) was obtained and analyzed in this study. The N. hirame circular mitogenome was 14,588 bp in size, consisting of 12 protein-coding genes (PCGs) lacking atp8 gene, 22 tRNA genes, 2 rRNA genes, 1 repeat region and several non-coding regions. The arrangement of PCGs was identical with those of other polyophistocotylean mitogenomes. The phylogenetic tree revealed that N. hirame clustered with Pseudochauhanea macrorchis, Neomazocraes dorsomatis, forming a sister group relationship to microcotylids.
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