Abstract
The complete mitochondrial genome sequence of Cylicocylus ashworthi was determined for the first time in the present study. The length of mtDNA sequences of C. ashworthi is 13,876 bp in size, containing 12 protein-coding genes (cox1-3, nad1-6, nad4L, atp6, and cytb), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and two non-coding regions. All genes are encoded by the same strand. The phylogenetic analysis by Bayesian inference method revealed that Cylicocylus genus was present in the same clade with high statistical support, and C. ashworthi was closely related with Cylicocylus nassatus than to other Strongylidae nematodes. The complete mt genome provide useful genetic marker for further studies of the taxonomy and systematics of Cyathostominae nematodes.
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