Abstract
BackgroundZucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits in Brazil. This virus belongs to the genus Tospovirus (family Bunyaviridae) and seems to be exclusively transmitted by Frankliniella zucchini (Thysanoptera). Tospoviruses have a tripartite and single-stranded RNA genome classified as S (Small), M (Medium) and L (Large) RNAS. Although ZLCV was identified as a member of the genus Tospovirus in 1999, its complete genome had not been sequenced until now.FindingsWe sequenced the full-length genome of two ZLCV isolates named ZLCV-SP and ZLCV-DF. The phylogenetic analysis showed that ZLCV-SP and ZLCV-DF clustered with the previously reported isolate ZLCV-BR09. Their proteins were closely related, except the non-structural protein (NSm), which was highly divergent (approximately 90 % identity). All viral proteins clustered similarly in our phylogenetic analysis, excluding that these ZLCV isolates have originated from reassortment events of different tospovirus species.ConclusionHere we report for the first time the complete genome of two ZLCV isolates that were found in the field infecting zucchini and cucumber.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0577-4) contains supplementary material, which is available to authorized users.
Highlights
Zucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits in Brazil
Here we report for the first time the complete genome of two ZLCV isolates that were found in the field infecting zucchini and cucumber
Some tospovirus species are notorious for their broadhost range [2], ZLCV mainly infects cucurbits and is only known to be transmitted by Frankliniella zucchini (Thysanoptera: Thripidae) [3]
Summary
Zucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits in Brazil. Conclusion: Here we report for the first time the complete genome of two ZLCV isolates that were found in the field infecting zucchini and cucumber. Even though ZLCV was described in 1999 [1] and some genes of one isolate has been sequenced [12,13,14], its complete genome is still unknown. We report the complete genome of two ZLCV isolates found infecting zucchini
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