Abstract
We determined the complete chloroplast genome sequence of Viola japonica using Illumina pair-end sequencing data. The whole chloroplast genome was 156,504 bp in length, consisting of a pair of inverted repeats (IR) of 26,427 bp, a large single-copy (LSC) region of 85,647 bp, and a small single-copy (SSC) region of 18,003 bp. The gene contents and order were exactly the same as published five Violaceae plastomes. A total of 111 unique genes were annotated, including 77 protein-coding genes, 30 tRNAs, and 4 rRNAs. Among the annotated genes, 16 genes contained one or two introns. The ML tree showed that V. japonica formed a clade with Viola seoulensis.
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