The Complete Chloroplast Genome Sequence of the Medicinal Plant Swertia mussotii Using the PacBio RS II Platform.

Beibei Xiang,Yong Wang,Jun Qian,Lizhi Wang,Lin Ma,Xiaoxuan Tian,Xiaoxue Li

doi:10.3390/molecules21081029

Abstract

Swertia mussotii is an important medicinal plant that has great economic and medicinal value and is found on the Qinghai Tibetan Plateau. The complete chloroplast (cp) genome of S. mussotii is 153,431 bp in size, with a pair of inverted repeat (IR) regions of 25,761 bp each that separate an large single-copy (LSC) region of 83,567 bp and an a small single-copy (SSC) region of 18,342 bp. The S. mussotii cp genome encodes 84 protein-coding genes, 37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. The identity, number, and GC content of S. mussotii cp genes were similar to those in the genomes of other Gentianales species. Via analysis of the repeat structure, 11 forward repeats, eight palindromic repeats, and one reverse repeat were detected in the S. mussotii cp genome. There are 45 SSRs in the S. mussotii cp genome, the majority of which are mononucleotides found in all other Gentianales species. An entire cp genome comparison study of S. mussotii and two other species in Gentianaceae was conducted. The complete cp genome sequence provides intragenic information for the cp genetic engineering of this medicinal plant.

Highlights

Swertia mussotii Franch (Zang Yin Chen, in Tibetan medicine) belongs to the family Gentianaceae.This species grows on the Qinghai Tibetan Plateau at an elevation of 3800–5000 m
Seven protein-coding, seven transfer RNA (tRNA), and all ribosomal RNA (rRNA) genes are duplicated in the inverted repeat (IR) regions
There were 45 SSRs in the S. mussotii cp genome (Table 6), the majority of which were mononucleotides (30) that we found in all the other species [40]

Summary

Introduction

Swertia mussotii Franch (Zang Yin Chen, in Tibetan medicine) belongs to the family Gentianaceae. This species grows on the Qinghai Tibetan Plateau at an elevation of 3800–5000 m. Several pharmaceutically-active compounds have been isolated and structurally identified from the whole S. mussotii plant, including oleanolic acid, ursolic acid, mangiferin, swertiamarin, and gentiopicroside [1,2,3,4]. Modern pharmacological research has demonstrated that these compounds have anti-hepatitis activity [5,6,7]. Due to the overexploitation of this plant, S. mussotii as a wild resource has become rare. S. mussotii seeds only germinate poorly when planted at low elevations

Methods

Results

Conclusion