Abstract

The complete chloroplast genome of Rosa banksiae var. normalis was reconstructed by reference-based assembly using Illumina paired-end data. The assembled plastome is 156,575 base pairs (bp) in length, including a pair of inverted repeat regions (IRs) of 26,008 bp each, a large single copy region (LSC) of 85,792 bp, and a small single copy region (SSC) of 18,767 bp. A total of 129 genes were predicted from the chloroplast genome, including 84 protein coding genes, 37 tRNA genes and 8 rRNA genes. The overall GC content of Rosa banksiae var. normalis chloroplast genome was 37.22%. Phylogenetic analysis with several reported chloroplast genomes showed that Rosa banksiae var. normalis is closely clustered with Rosa odorata var. gigantea, Rosa multiflora, Rosa chinensis var. spontanea, and Rosa roxburghii. The complete chloroplast genome of Rosa banksiae var. normalis provides new insight into Rosaceae evolutionary and genomic studies.

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