Abstract
Clerodendranthus spicatus (Thunb.) C. Y. Wu ex H. W. Li is one of the most important medicines for the treatment of nephrology in the southeast regionsof China. To understand the taxonomic classification of Clerodendranthus species and identify species discrimination markers, we sequenced and characterized its chloroplast genome in the current study. Total genomic DNA were isolated from dried leaves of C. spicatus and sequenced using an Illumina sequencing platform. The data were assembled and annotated by the NOVOPlasty software and CpGAVAS2 web service. The complete chloroplast genome of C. spicatus was 152,155bp, including a large single-copy region of 83,098bp, a small single-copy region of 17,665bp, and a pair of inverted repeat regions of 25,696bp. The Isoleucine codons are the most abundant, accounting for 4.17% of all codons. The codons of AUG, UUA, and AGA demonstrated a high degree of usage bias. Twenty-eight simple sequence repeats, thirty-six tandem repeats, and forty interspersed repeats were identified. The distribution of the specific rps19, ycf1, rpl2, trnH, psbA genes were analyzed. Analysis of the genetic distance of the intergenic spacer regions shows that ndhG-ndhI, accD-psaI, rps15-ycf1, rpl20-clpP, ccsA-ndhD regions have high K2p values. Phylogenetic analysis showed that C. spicatu is closely related to two Lamiaceaespecies, Tectona grandis, and Glechoma longituba. In this study, we sequenced and characterized the chloroplast genome of C. spicatus. Phylogenomic analysis has identified species closely related to C. spicatus, which represent potential candidates for the development of drugs improving renal functions.
Highlights
Clerodendranthus spicatus (Thunb.) C.Y.Wu, commonly called as Yanumiao, Maoxugong and Maoxucao [1]
C. spicatu was closely related to the genus of two Salvia speices, Tectona grandis, Cistanche deserticola and Glechoma longituba belonged to the Lamiales
Assembly and annotation of C. spicatus chloroplast genome based on the next-generation sequencing technology
Summary
Clerodendranthus spicatus (Thunb.) C.Y.Wu, commonly called as Yanumiao, Maoxugong and Maoxucao [1]. It is a kind of perennial herbs and is the first reported among Clerodendranthus genus in the Lamiaceae family. It is mainly produced in the area of Guangdong, Hainan, Guangxi, Yunnan, Fujian province and Taiwan province. It is distributed in India, Myanmar, Thailand, Indonesia, Philippines to Australia and adjacent islands [2]. The entire plants or aerial parts are widely used in the treatment of chronic nephritis, cystitis, lithangiuria and rheumatoid arthritis, which hold good effects on the kidney disease [10,11]
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