Abstract
Jerusalem artichoke (Helianthus tuberosus L.) is widely cultivated in Northwest China, and it has become an emerging economic crop that is rapidly developing. Because of its elevated inulin content and high resistance, it is widely used in functional food, inulin processing, feed, and ecological management. In this study, Illumina sequencing technology was utilized to assemble and annotate the complete chloroplast genome sequences of Jerusalem artichoke. The total length was 151,431 bp, including four conserved regions: A pair of reverse repeat regions (IRa 24,568 bp and IRb 24,603 bp), a large single-copy region (83,981 bp), and a small single-copy region (18,279 bp). The genome had a total of 115 genes, with 19 present in the reverse direction in the IR region. A total of 36 simple sequence repeats (SSRs) were identified in the coding and non-coding regions, most of which were biased toward A/T bases. A total of 32 SSRs were distributed in the non-coding regions. A comparative analysis of the chloroplast genome sequence of the Jerusalem artichoke and other species of the composite family revealed that the chloroplast genome sequences of plants of the composite family were highly conserved. Differences were observed in 24 gene loci in the coding region, with the degree of differentiation of the ycf2 gene being the most obvious. A phylogenetic analysis showed that H. petiolaris subsp. fallax had the closest relationship with Jerusalem artichoke, both members of the Helianthus genus. Selective locus detection of the ycf2 gene in eight species of the composite family was performed to explore adaptive evolution traits of the ycf2 gene in Jerusalem artichoke. The results show that there are significant and extremely significant positive selection sites at the 1239N and 1518R loci, respectively, indicating that the ycf2 gene has been subject to adaptive evolution. Insights from our assessment of the complete chloroplast genome sequences of Jerusalem artichoke will aid in the in-depth study of the evolutionary relationship of the composite family and provide significant sequencing information for the genetic improvement of Jerusalem artichoke.
Highlights
Jerusalem artichoke (Helianthus tuberosus L.) is a species of the composite family native to North America, primarily distributed in the temperate zone of 40–55 C north latitude and the temperate region with the approximate similar latitude in the southern hemisphere
The chloroplast genome of Jerusalem artichoke had a total guanine-cytosine content (GC content) of 37.6%, with GC in the IR region corresponding to 43.2%, and GC in the LSC and SSC regions being 35.6% and 31.3%, respectively
The chloroplast genome of Jerusalem artichoke contained 115 genes, including 84 protein-coding genes CDS, 27 tRNA genes and four rRNA genes distributed in the IR region
Summary
Jerusalem artichoke (Helianthus tuberosus L.) is a species of the composite family native to North America, primarily distributed in the temperate zone of 40–55 C north latitude and the temperate region with the approximate similar latitude in the southern hemisphere. Jerusalem artichoke was introduced to China via Europe in the 17th century. It has been grown on a small scale as a pickled vegetable in various regions of China. Jerusalem artichoke is highly resistant and can be grown in saline, alkaline, dry, and low temperature conditions. It is widely cultivated in various regions of China, especially in the Qinghai plateau in recent years. Most research on Jerusalem artichoke has focused on ecological management, feed research and development, and the processing of inulin products. Jerusalem artichoke utilizes fructan as a source of carbon, instead of starch, as most crops. Fructan can be processed or modified, providing the raw materials for the production of bioethanol, paper, and healthcare products (Saengkanuk et al, 2011; Wang et al, 2015; Wyse, Young & Jones, 2017)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
