Abstract
Rheum nobile is a model species for investigating alpine adaptation in ‘glasshouse’ plants. In this study, the complete chloroplast (cp) genome of R. nobile was assembled based on next-generation sequencing. The cp genome was 161,250 bp in length, including a large single copy (LSC) region of 86,290 bp, a small single copy (SSC) region of 12,838 bp and a pair of inverted repeats (IRs), each of 31,061 bp. The genome contained 127 genes, including 82 protein-coding genes, 35 tRNA genes and 8 ribosomal RNA genes. Most of the protein-coding genes contained a single exon; only 10 genes contained two exons and 4 gene had three exons (i.e., ycf3). The overall GC content of the chloroplast genome was 37.29%, while the corresponding values for the LSC, SSC, and IR regions were 35.33%, 32.39% and 41.03% respectively. Phylogenetic analysis showed that R. nobile clustered with the other three Rheum species included in the study, and Rheum is closely related to Oxyria.
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