Abstract
Menispermum dauricum is a woody liana with great medicinal value. In the current study, we assembled the first chloroplast (cp) genome of M. dauricum. The whole chloroplast genome is 158,623 bp in length, with one large copy region (LSC: 88,879 bp), a small single copy region (SSC: 20,644 bp), and two inverted repeats (IR: 24,550 bp). The cp genome contains 114 unique genes with 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. In our phylogeny of Ranunculales, Papaveraceae is found to be the basal group of Ranunculales and M. dauricum is sister to Stephania japonica.
Highlights
Menispermum dauricum is a woody liana with great medicinal value
The rps12 gene is trans-spliced, with the 50 end located in the LSC and the 30 end duplicated in the inverted repeats (IR) regions
Maximum likelihood analysis was carried out using RAxML-HPC2 on XSEDE v8.2.10 [AQ1] (Stamatakis 2014)
Summary
Menispermum dauricum is a woody liana with great medicinal value. In the current study, we assembled the first chloroplast (cp) genome of M. dauricum. The Asian moonseed (Menispermum dauricum DC.) is a woody liana widely distributed in East Asia from Central China to Siberia, Korean Peninsula, and Japan. Genomic data for this important medicinal plant are scarce. We sequenced the complete chloroplast genome of M. dauricum and analysed its phylogenetic position in Ranunculales.
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