Abstract
Medicago sativa cv. Hangmu No.1 is the first variety of alfalfa bred by space mutation breeding in China, which characterized by high multifoliate rate, high yield, and abundant nutrients. To some extent, this space mutation alfalfa will efficiently solve the severe shortage of forage and contribute to rapid development in animal husbandry in China. In this study, the complete chloroplast genome sequence of M. sativa cv. Hangmu No.1 was evaluated based on Illumina Hiseq 2500 platform. The circular genome was 123,825 bp in length, containing 102 genes including 75 protein-coding genes (70 PCG species), 24 transfer RNA genes (21 tRNA species) and 3 ribosomal RNA genes (3 rRNA species). Consistent with other Medicago chloroplast genomes, the M. sativa cv. Hangmu No.1 genome did not contain inverted repeat (IR) regions. Phylogenetic analysis based on 11 chloroplast genomics indicates that M. sativa cv. Hangmu No.1 is closely related to M. sativa and Medicago falcata strain. This complete chloroplast genome will provide valuable information for the molecular breeding of M. sativa.
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