Abstract
Fraxinus pennsylvanica is distributed in the central and eastern USA and Canada. It is assessed as Critically Endangered (CR). In this study, the complete chloroplast (cp) genome sequence of F. pennsylvanica was determined using next-generation sequencing. The entire cp genome was determined to be 155,543 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 86,389 and 17,806 bp, respectively, which were separated by a pair of 25,674 bp inverted repeat (IR) regions. The genome contained 132 genes, including 87 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The overall AT content of the genome is 37.8%. A phylogenetic tree reconstructed by 10 chloroplast genomes reveals that F. pennsylvanica is most related with F. chiisanensis.
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