Abstract

Akebia trifoliata subsp. australis (Diels) T.Shimizu is a medicinal plant in China. Here, the complete chloroplast (cp) genome sequence of A. trifoliata subsp. australis was assembled and characterized as a resource for future genetic studies. The whole cp genome was 157,952 bp in length, containing of a large single-copy (LSC) region of 86,596 bp, a small single-copy (SSC) region of 19,060 bp, and two inverted repeat (IR) regions of 26,148 bp. The new sequence possessed total 113 unique genes, including 79 protein-coding genes, 30 tRNA genes and 4 rRNA genes. The nucleotide composition was asymmetric (30.3% A, 19.7% C, 19.0% G and 31.0% T) with an overall GC content of 38.7%. The maximum likelihood phylogenetic analysis based on 10 cp genomes indicated that A. trifoliata subsp. australis was closely related to Akebia trifoliata subsp. trifoliata. However, Akebia quinata was closely related to Stauntonia obovatifoliola.

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