The complete chloroplast genome and characteristics analysis of Musa basjoo Siebold

Fenxiang Liu,Dezhi Xu,Yu Zhang,Wenguo Yang,Chuanbei Jiang,Ali Movahedi,Jigang Xie

doi:10.1007/s11033-021-06702-5

Abstract

An ornamental plant often seen in gardens and farmhouses, Musa basjoo Siebold can also be used as Chinese herbal medicine. Its pseudostem and leaves are diuretic; its root can be decocted together with ginger and licorice to cure gonorrhea and diabetes; the decoct soup of its pseudostem can help relieve heat, and the decoct soup of its dried flower can treat cerebral hemorrhage. There have not been many chloroplast genome studies on M. basjoo Siebold. We characterized its complete chloroplast genome using Novaseq 6000 sequencing. This paper shows that the length of the chloroplast genome M. basjoo Siebold is 172,322bp, with 36.45% GC content. M. basjoo Siebold includes a large single-copy region of 90,160bp, a small single-copy region of 11,668bp, and a pair of inverted repeats of 35,247bp. Comparing the genomic structure and sequence data of closely related species, we have revealed the conserved gene order of the IR and LSC/SSC regions, which has provided a very inspiring discovery for future phylogenetic research. Overall, this study has constructed an evolutionary tree of the genus Musa species with the complete chloroplast genome sequence for the first time. As can be seen, there is no obvious multi-branching in the genus, and M. basjoo Siebold and Musa itinerans are the closest relatives.

Highlights

Musa basjoo Siebold, a perennial herb of the family Musaceae belonging to the genus Musa, is one of the main tropical plants
The plastid genome structure of Musa basjoo Siebold shows a typical quadripartite circular molecule (Fig. 1), including a large single copy (LSC; 90,160 bp) and a short single copy (SSC; 11,668 bp), which is divided into a pair of inverted repeats (IRs; 35,247 bp) regions (Fig. 1 and Table S1)
The genome sequencing, assembly, annotation, and comparative analysis revealed that Musa basjoo Siebold cp genomes had a typical quadruple structure with a conserved arrangement

Summary

Introduction

Musa basjoo Siebold, a perennial herb of the family Musaceae belonging to the genus Musa, is one of the main tropical plants. It is mainly distributed in subtropical areas in China, Guizhou, Guangdong, Guangxi, Hainan, Sichuan, Yunnan, and Taiwan (Yang et al 2013; Amano et al 1991). Its flowers, leaves, and roots have high medicinal values They are mainly used to treat rheumatism and other cardiovascular, cerebrovascular, digestive, and circulatory systems (Xu et al 2014; Morimoto et al 2008; Balthasar et al 2005; Gupta et al 2004; Han et al 2013; Kim et al 2012). Triterpenoids, xanthones, and alkaloids (Otálvaro et al 2002; Pascual-Villalobos and Rodríguez 2007; Tamura 1998)

Methods

Results

Discussion

Conclusion