Abstract
Physiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.
Highlights
The balance between pro- and antioxidative processes is crucial for proper functioning of all cells and tissues
The balance between production and neutralization of free radicals is a part of physiological, aerobic metabolism of cells
Placental tissues were collected in local slaughterhouse from healthy HF cows (n = 8) in 2–3 month of pregnancy
Summary
The balance between pro- and antioxidative processes is crucial for proper functioning of all cells and tissues. Among others, are target molecules for free radicals Their peroxidative damage may influence important processes and alter physiological functions of proteins including enzyme and hormone action or properties of structural proteins [4]. As this damage may affect selected aminoacids in a protein chain, one of the methods for its estimation is the determination of bityrosine bridges [5]. Peroxidative damage to lipid molecules leads to fatty acid fragmentation and formation of radicals.
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