The comparison of biological characteristics of mesenchymal stem cells derived from different placenta tissues

Abstract

Objective To analyze the biological characteristics and differentiation potential of mesenchymal stem cells derived from different placenta tissues.Methods We established a system to amplify amniotic mesenchymal stem cells (AMSCs) and chorionic mesenchymal stem cells(CMSCs) in vitro and analyzed the cell phenotype of the two groups of mesenchymal stem cells (MSCs) by flow cytometry.We observed the distribution of CMSCs in chorionic and the expression of programmd death ligand-1 (PD-L1) by immunohistochemistry.We also detected the expression of neuron-specific protein in the two groups of MSCs before and after induction by RT-PCR.Results Both MACs and CMSCs expressed CD90,CD73,CD105 in high level,but not CD14,CD34,CD45,HLA-DR.Costimulatory molecule PD-L1 was found in CMSCs,but not in AMSCs.The CMSCs with positive expression of CD90 and CD166 were located in close proximity to the trophoblast cells.Before induction,Musashi-1,Nestin,β-tubulin Ⅲ (Tuj1) and GFAP mRNA expression were found in AMSCs,while only Nestin mRNA was detected in CMSCs.2 days after induction,musashi-1,nestin,β-tubulin Ⅲ,glil fibrillary acidic protein (GFAP),neurafilament protein (NF) mRNA expression was also detected in AMSCs,while Nestin could be detected in CMSCs.5 days after induction,the neural-specific protein expression pattern in AMSCs was almost the same as that at 2 days after induction except declined Nestin expression,while NF and GFAP expression could be detected in CMSCs.Conclusion AMSCs have good neurobiology properties and are easier to differentiate to neurons or glial cells under certain conditions.CMSCs could be the better source of cells to study the immunomodulatory mechanism of MSCs. Key words: Amniotic mesenchymal stem cells; Chorionic Mesenchymal stem cells; Programmd death ligand-1 ; Neuron-specific protein