The Comparison between the Yellow and Pink Flowers Harvested from Hainan on the Vegetable Proteins and Metallic Elements

Abstract

In this study, the yellow and pink flowers of  Lantana camara L.  harvested from Hainan were used as the experimental samples, two-dimensional electrophoresis (2-DE) based on BPP + phenol extraction was used to separate and extract the proteins from the yellow flower and pink flowers harvested from Hainan  Lantana camara  L. under the optimized conditions. The gel images of the well-expressed protein were obtained, which were analyzed by using Image Master5.0 software. Then the corresponding enzymatic hydrolysis products were identified by MALDI-TOF-MS. 23 kinds of the differentially expressed proteins from the yellow flowers and 25 kinds from pink flowers of  Lantana camara  L .  were determined by means of bioinformatics. There were 9 kinds of common proteins, 6 kinds of peculiarly expressed proteins in yellow flower and 7 kinds of peculiarly expressed proteins in pink flower, respectively. In addition, a microwave digestion inductively coupled plasma mass spectrometry (ICP-MS) was performed to analyze 25 kinds of metal elements and their contents including K, Ca, Mg, Na, Al, Fe, Mn, Cu and Zn etc. The result indicated that the contents of K, Al, Mg and Fe are higher. Based on the above experimental results, it is the first time to measure the types and contents of vegetable proteins and metal elements contained in the flowers of  Lantana camara  L.. It is possible for further explanations on its pharmacological activity as a botanical drug from the point of proteins and metal elements, which provides a theoretical guidance for the further development and utilization of Hainan characteristic economic crops.