Abstract
Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differences can affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semen of four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods. Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, free sulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods before oxidative and antioxidative status analyses significantly affected the result (P < 0.05), and the ultrasonic homogenization method was the most effective. It is recommended to use the ultrasonic homogenization as a pretreatment method to determine the oxidative and antioxidative status analyses in sperm.
Highlights
Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies
The pretreatment protocols before oxidative analyses can be categorized as direct oxidative analyses [2,3,4], washing and chemical treatment[5], rotary homogenization [6], and sonication [7]
Oxidative status of sperm samples is assessed by measuring the levels of serum lipid hydroperoxide (LOOH) and total oxidant status (TOS)
Summary
Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. Pretreatment procedure of semen before oxidative analyses is contradictory in different studies. The rotary, bead, and ultrasonic homogenization methods were tested in terms of the effectiveness of the analysis of the oxidative and antioxidative status of semen. The data was analyzed to see if there were statistical differences between homogenization methods and oxidative status parameters.
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