The Comparative Analysis of Anti-aging Indicators of Oenothera laciniata, Equisetum arvense and Ailanthus altissima Extracts in D-Galactose-Induced Aging Animal Model

Abstract

Abstract Objectives The previous in vitro studies reported research has identified that the leaves extracts of Oenothera laciniata, Equisetum arvense and Ailanthus altissima presented the high anti-oxidative effects. This study was performed to comparatively measure the in vivo anti-aging functional effect of these three samples in d-galactose-induced aging model. Methods The 16-week-old rats were randomly divided into 3 groups (n = 5): d-galactose + Oenothera laciniata (O group); d-galactose + Equisetum arvense (E group); d-galactose + Ailanthus altissima (A group). All groups were treated with d-galactose by intra-peritoneally injection and oral gavage administration of 3 extract samples with either 300 mg/kg or 100 mg/kg for 8 weeks. Results After sacrifice the common aging indicators, MDA(Malondialdehyde) and AGEs(Advanced glycation end-products) were primarily measured in serum and liver tissue. The MDA concentration was presented in the order of E ≥ O > A in serum (n = 5) and O ≥ A > E in liver tissue (n = 4). The AGEs concentration was presented not significantly different in serum (n = 5) and O = A > E in liver tissue (n = 3). Conclusions The present data showed that Equisetum arvense may have the highest anti-aging effect in vivo among three samples treated by diminishing MDA and AGEs in liver. The analysis of protein expression of PI3K/Akt and tissue measurement of NO level are currently under investigation. Funding Sources This study was supported by Ministry of Trade, Industry and Energy, Republic of Korea.