Abstract
Abstract Objectives The previous in vitro studies reported research has identified that the leaves extracts of Oenothera laciniata, Equisetum arvense and Ailanthus altissima presented the high anti-oxidative effects. This study was performed to comparatively measure the in vivo anti-aging functional effect of these three samples in d-galactose-induced aging model. Methods The 16-week-old rats were randomly divided into 3 groups (n = 5): d-galactose + Oenothera laciniata (O group); d-galactose + Equisetum arvense (E group); d-galactose + Ailanthus altissima (A group). All groups were treated with d-galactose by intra-peritoneally injection and oral gavage administration of 3 extract samples with either 300 mg/kg or 100 mg/kg for 8 weeks. Results After sacrifice the common aging indicators, MDA(Malondialdehyde) and AGEs(Advanced glycation end-products) were primarily measured in serum and liver tissue. The MDA concentration was presented in the order of E ≥ O > A in serum (n = 5) and O ≥ A > E in liver tissue (n = 4). The AGEs concentration was presented not significantly different in serum (n = 5) and O = A > E in liver tissue (n = 3). Conclusions The present data showed that Equisetum arvense may have the highest anti-aging effect in vivo among three samples treated by diminishing MDA and AGEs in liver. The analysis of protein expression of PI3K/Akt and tissue measurement of NO level are currently under investigation. Funding Sources This study was supported by Ministry of Trade, Industry and Energy, Republic of Korea.
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