Abstract
Oligopeptides subjected to partial enzyme hydrolysis provide mixtures of sub-peptides which optimize amino acid sequence determination by fast atom bombardment mass spectrometry. The digestion mixture can be sampled directly; there is no interference from the enzyme. Complete sequences were obtained from spectra recorded on 1–5 nmol samples after digestion at 37°C for times ranging from 0.1–4.0 h. The qualitative results of attack by two exopeptidases on three oligopeptides are discussed.
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