The Combined Effects of Material Surface Chemistry and Vitamin D3 on MC3T3-E1 Cells

Abstract

Separate stimulation by vitamin D3 and material surface chemistry has long witnessed and proved remarkable effects on biological behavior of osteoblasts, but combined stimulation effects of these two have not been reported yet. In this project, the surface of hydroxyl, amino and methyl groups were constructed by silicon-alkyl self-assembly technology. Then, chemical surfaces were to be integrated with vitamin D3 at different concentrations (10−7 mol/L, 10−8 mol/L, 10−9 mol/L) to stimulate MC3T3-E1 osteoblasts. It turned out to be quite successful in establishing the research platform for combined stimulation of material surface chemistry and vitamin D3. Meanwhile, indicators such as osteoblast adhesion spots, cytoskeleton formation, proliferation, and differentiation have been fully examined in the process for us to explore general rules and possible mechanism of combined stimulation effects of these two on early responses of osteoblasts. The results suggest that the combined effects of vitamin D3 and material surface chemistry can induce early responses of osteoblasts on different material surfaces, with differing response degrees. Combined stimulation of material surface chemistry-vitamin D3 causes adhesion plaque and cytoskeleton differences, leading to different early cell behaviors. These findings will provide a strong theoretical basis for the treatment of osteoporosis and the construction of tissue-engineered bone models in vitro.