Abstract
Hyperglycemia conditions increase free radicals in the body that cause oxidative stress. Oxidative stress increase lipid peroxidation activity and reactive oxygen species (ROS). An antioxidant can prevent a free radical movement. The materials that contain potent antioxidants are black soybeans tempeh (BST) and purple sweet potatoes (PSP). The antioxidants in the BST are isoflavones with their derivates, and PSP is anthocyanins. This study aimed to determine the effect of BST and PSP extract on reactive oxygen species (ROS) and malondialdehyde (MDA) levels. In this study, rats were given a high-fat diet, 10% sucrose drink, and injected with multiple low-dose streptozotocin to induce T2DM. The animal's experiment divided into six groups: healthy rats, DM rats, DM rats + glibenclamide, DM rats + combination of BST and PSP in 3:1, 1:1, and 1:3 respectively. ROS levels were determined using the ELISA method and MDA levels were determined using spectrophotometer according to Thiobarbituric Acid (TBA) method. Our result suggests that the combination of BST and PSP significantly reduces ROS and MDA levels.
Highlights
Diabetes mellitus is characterized by chronic hyperglycemic conditions (Baynest, 2015)
Oxidative stress increases the oxidation of glucose, amino acids, and lipids, which affect the production of Malondialdehyde (MDA), and Reactive oxygen species (ROS) that trigger DNA damage (Sakuraba et al, 2002)
Our result suggest that reactive oxygen species (ROS) level increase significantly in DM rats compared to normal rats (Figure 1)
Summary
Diabetes mellitus is characterized by chronic hyperglycemic conditions (Baynest, 2015). 425 million people aged 20-79 had diabetes in 2017 This number is expected to increase to 625 in 2045 (Cho et al, 2018). Oxidative stress increases the oxidation of glucose, amino acids, and lipids, which affect the production of Malondialdehyde (MDA), and Reactive oxygen species (ROS) that trigger DNA damage (Sakuraba et al, 2002). Purple sweet potato abundant anthocyanin that one of the potent antioxidants (Gras et al, 2017). Anthocyanin works by increasing the workings of insulin receptors, improving antioxidant status by suppressing MDA production, and enhancing Superoxide Dismutase (SOD) levels. This study used a combination of an extract of black soybean Tempe and purple sweet potato. There no research of the combination of black soybean and purple sweet potato extract in overcoming type 2 diabetes mellitus
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