Abstract
An insectary colony of Anopheles litoralis King, a suspected secondary malaria vector in the Philippines, has been successfully established in Manila. Enough eggs were obtained from 329 wild-caught blooded females to start the F1 generation. Artificial mating was used to inseminate the females. Observations on the copulation times, insemination rates, and blood meal requirements for nullipars, as well as detailed records on the first 6 generations, are given. Since the species is a salt-water, coastal breeder, saline water was used for rearing the immature stages and for the oviposition medium. Satisfactory insemination, oviposition, and larval rearings were obtained and the colony is now in the 19th generation and continues vigorously.
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