The collaborative role of NSE and S-100 in the pathogenesis of congenital biliary dilatation

Abstract

Objective To explore the role and clinical significance of NSE and S-100 in the pathogenesis of congenital biliary dilatation (CBD). Methods From 2007 to 2009,36 children with CBD underwent choledochal cyst excision and hepaticojejunostomy. The proximal cyst walls, distal cysts walls and gallbladder walls were collected as experimental group. At the same time, the extrahepatic bile ducts and gallbladders of 15 fetus who were late terminated because of unplanned pregnancy were selected as the control group. Then the specimens of two groups were fixed, embedded, and immunohistochemical stained. In different parts of biliary tracts, the expressions and distributions of NSE and S-100 were compared and the relation were analyzed between the diameters of cysts and the expressions of NSE and S-100. The results were statistically analyzed by SPSS12.0 for windows software.Results The expressions of NSE and S-100 in the distal cyst (1.86 ± 1.29, 1.81 ± 1.04) were weaker than those in the proximal ones (3. 61 ± 1.92, 3. 58 ± 1.95) (P＜0. 05). The expressions of these two proteins in gallbladder (3. 42 ± 1.99 vs 3.72 ± 2. 08) were also significant lower than those in the distal cyst (P＜0. 05), but no difference could be noted between the proximal cyst and gallbladder. There were no significant difference of these two proteins between fetal bile duct(4. 86 ± 2. 97, 5. 14 ± 2. 73)and fetal gallbladder (3. 71 ± 2. 14, 4. 00 ± 1.63) (P＞0. 05) . The expressions of NSE and S-100 in distal cysts were also lower than those of fetal bile ducts and gallbladders(P＜0. 05). The diameter of cysts and the expressions of NSE and S-100 in different parts of biliary tracts were all negatively correlated each other (P＜0. 05). Conclusions The impaired distributions of ganglion cells and nerve fibers in the distal bile duct are related to the pathogenesis of CBD. The development of ganglion cells and nerve fibers are negatively related to the size of cysts. The collaborative role of NSE and S-100 can provide theoretical basis for the right range of cyst excision. Key words: Choledochal cyst; S100 proteins