The cold-induced denaturation of lactate dehydrogenase at sub-zero temperatures in the absence of perturbants

Abstract

The cold-induced denaturation oflactate dehydrogenase has been determined in an unfrozen, cryoprotectant-free solution at sub-zero temperatures. The cold-induced denaturation temperature ( T l has been found to be −28°C. The results for the first time clearly establish that temperature alone can induce denaturation in a cooled protein solution. The validity of earlier data, obtained in the presence of perturbants (particularly pH or guanidinium chloride), is discussed. Lactate dehydrogenase; Enzyme denaturation, cold; pH; Temperature; Cryosolvent