Abstract
Chemical analyses (amino acid composition coupled with peptide mapping), gel chromatography and SDS-polyacrylamide gel electrophoresis were used to determine the molecular weights of the coat protein subunits of several strains of cucumber viruses 3 and 4 (CV3 and CV4). The results obtained by means of amino acid analyses and peptide mapping were 17,100 for two strains of CV3 and 16,100 for two strains of CV4. These values are considered accurate to within ±1%, and if they are considered to be the actual values, the results obtained on these same strains of CV3 and CV4 by C-terminal analyses, SDS-polyacrylamide gel electrophoresis, and gel chromatography show deviations ranging from −0.6% to +47%. Gel chromatography in guanidine hydrochloride appeared to be next in reliability to amino acid analyses and peptide mapping.
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