Abstract
The cluster Re6Se8I63− has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25–150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63− is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63− could be intravenously administered in animals at therapeutic doses for in vivo studies.
Highlights
Success in cancer chemotherapy is based on the selectivity of some drugs to induce tumor cell death without affecting normal cells
Erythrocytes exposed to 100–150 μM of the cluster showed echinocytosis and crenation
We examined the hemolytic properties of increasing doses of the cluster Re6Se8I63− in human erythrocytes in vitro, finding low hemolysis levels at therapeutic concentrations
Summary
Success in cancer chemotherapy is based on the selectivity of some drugs to induce tumor cell death without affecting normal cells. OCCH2(CH2CH2O)12OCH3 complex are internalized into cells and are mainly localized in the cytoplasm and nucleus These clusters induce the suppression of cell proliferation and viability at concentrations higher than 100 μM with no cytotoxic effects at physiological concentrations (50 μM). Recent studies have shown that the K4[Re6S8(CN)6] cluster complex is mainly accumulated in the liver and can be excreted by the kidneys in rats. It was accumulated in the spleen in significant amounts [5]. The potential protective role of albumin was evaluated
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