The cloning and overproduction of Escherichia coli uracil-DNA glycosylase

Abstract

Escherichia coli cells containing elevated levels of the DNA repair enzyme uracil-DNA glycosylase (the ung gene product) have been constructed by in vitro recombination methods. First, λ nadB transducing phages were isolated from two E. coli DNA libraries by selection of nicotinate-independent lysogens. λ nadB phage from one of the libraries were also ung + and carried the ung-nadB genes on an 8.3 kb HindIII restriction fragment. The ung and nadB genes were subcloned into plasmids and a restriction map of the ung region of the E. coli chromosome was constructed. The uracil glycosylase gene was localized to a 1.4-kb restriction fragment by subcloning the gene into pBR322. Uracil glycosylase was overproduced (relative to the specific activity of wild type cells) by about two-fold in λ ung lysogens and by 15- to 20-fold in cells containing pBR322 ung derivatives. When the ung gene and its promoter were placed downstream from the bacteriophage λp L promoter in the plasmid pKC30, uracil glycosylase production was heat-induced to more than 100-fold above the levels of a wild-type cell. By relating the insertion orientation of the λ ung gene in the plasmid pKC30 to its orientation in λ ung-nadB transducing phages, the transcription direction of the ung gene on the E. coli linkage map was found to be clockwise.