Abstract
Vibrio eltor phage e 4 codes for five different tRNA species (S. Chattopadhyay and R. K. Ghosh, 1988, Virology, 165, 606–608) . The tRNA genes contained in a 3.4-kb Kpnl fragment (S. Chattopadhyay and R. K. Ghosh, 1988, Virology, 162, 337–345) have been cloned in pUC 19 at the Kpnl site. Two recombinant plasmids, pSR216 and pSR112, produced four of the five tRNA species (arginine, isoleucine, tyrosine, and tryptophan) encoded in the phage genome. The tRNA genes were located on a 1.45-kb Kpn1- HindIII subfragment.
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