Abstract
BackgroundWe aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer.MethodsMicroRNA microarray was used to screen differentially expressed microRNAs between the chemosensitive and chemoresistant epithelial ovarian cancer tissues. RT-PCR was used to validate the candidate microRNA. The potential target genes and their enriched biological pathways of microRNA were also analyzed. Dual Luciferase Reporter Gene Assay was conducted to validate the regulation of miRNA-1307 on the 3’-UTR of DAPK3.ResultsmiRNA-1307 was up-regulated in the chemoresistant epithelial ovarian cancer tissues compared to the chemosensitive counterparts. The up-regulation of miRNA-1307 was not associated with menopause, tumor differentiation state, clinical stage, and lymph node metastasis of ovarian cancer. Gene ontology analysis of miR-1307 candidate target genes indicated that miR-1307 candidate target genes were enriched in the processes of cell proliferation and differentiation, nucleotide synthesis and metabolism, and lymphocytes activation.ConclusionOur results suggest that miRNA-1307 may play a role in the development of chemoresistance in ovarian cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s13048-015-0143-5) contains supplementary material, which is available to authorized users.
Highlights
Epithelial ovarian carcinoma has the highest mortality rate in gynecological cancers
Upregulation of miR-1307 in chemoresistant ovarian tumors To search for differentially expressed miRNA that may play a role in the development of chemoresistance in ovarian cancer, we compared the miRNA expression profile between 4 chemosensitive and 4 chemoresistant ovarian tumor samples
We discovered that miR-1307 was upregulated in chemoresistant ovarian tumors when compared to the chemosensitive ovarian tumors (Figure 1)
Summary
Epithelial ovarian carcinoma has the highest mortality rate in gynecological cancers. 52% of death caused by gynecological cancer are contributed by the epithelial ovarian carcinoma [1]. The current standard treatment strategy for epithelial ovarian carcinoma is cytoreductive surgery followed by platinum-based chemotherapy. Despite the advancements in the diagnosis and treatment of ovarian cancer, the 5-year survival rate for advanced ovarian cancer is poorly 30% [2].The main reasons for the high mortality of ovarian cancer include: early stage of ovarian cancer are usually asymptomatic; lack of diagnostic methods for early stage of ovarian cancer; and the development of chemoresistance during chemotherapy [3]. Past studies have shown that chemoresistance occurred in 1/3 of patients received platinum-based chemotherapy. Most recurrent ovarian tumors are resistant to platinum-. We aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
