The clinical value of miR–127–3p expression in plasma of patients with breast cancer

Abstract

Objective To investigate the expression and clinical value of miR–127–3p in plasma of patients with breast cancer. Methods 80 cases of breast patients, 70 cases of benign breast tumor patients and 70 cases of normal control group were recruited. A real–time fluorescent quantitative reverse transcription polymerase chain reaction (RT–PCR) method for detecting miR–127–3p was established; Liner, reproducibility, and specificity were evaluated. In addition, correlations between the relative expression of plasma miR–127–3p and the concentrations of CEA and CA153 were assessed. the relationship of miR–127–3p expression and clinicopathological features was further determined by Mann–Whitney test. Results The method for detection of plasma miR–127–3p was established. The relative plasma expression of miR–127–3p in breast patients [10.561 (5.424–16.465)] was significantly higher than that in benign breast tumor patients [3.015 (1.987–5.035)] (P=0.000 6) and healthy controls [2.375 (1.173–4.370)] (P=0.000 2). However, there was no significant difference between benign tumor patients and healthy control group (P=0.143). Positive relationship was found between the relative expression of miR–127–3p and the concentration of CA153 (R2=0.457, P=0.003). The area under the ROC curve(AUC) of miR–127–3pwas 0.763, which was higher than that of CEA and CA153. No significant difference was found between plasma miR–127–3p expression and clinicalpathological features including tumor size, differentiation and tumor node metastasis stage (P>0.05). Conclusions miR–127–3p was increased in breast cancer patients and may be an important diagnostic index for breast cancer.(Chin J Lab Med, 2015, 38: 682–685) Key words: Breast neoplasms; microRNAs; Reverse transcriptase polymerase chain reaction