The clinical study of pre-and post-operative sequential changes of serum p53 antibodies in patients with esophageal squamous cell cancer

Abstract

Objective To explore the relationship between serum p53 antibodies and clinicopathological characteristics in patients with esophageal squamous cell carcinoma(ESCC) and to investigate sequential changing regularity of serum p53 antibodies before and after surgical resection. Methods The eligible ESCC cases of this study were consecutively recruited between October 2010 and February 2012 from Luhe Hospital.There were 68 males and 30 females.The serum p53 antibody concentration was detected in ESCC patients and in 30 healthy adults as controls by enzyme linked immunosorbent assay(ELISA).The blood samples were collected on the day before operation and on the 7th, 30th, 90th and 180th days postoperatively. Results (1) The concentration[(338. 96± 104. 14)ng/L vs.(242. 30±39. 79) ng/L]and positive rate(37.8% vs.0)of serum p53 antibodies in patients with ESCC were significantly higher than normal individuals(P< 0. 05).(2) The positive rate of pre-operative serum p53 antibodies in patients with ESCC positively correlated with the pieces of consumed cigarette, cell differentiation and TNM(P< 0. 05).(3) The level of serum p53 antibodies decreased gradually in patients with ESCC underwent radical resection of the cancer, and completely returned to the normal level around the 30th day after operation. Conclusion (1)Serum p53 antibody can be used as a potential marker for early detection of ESCC and predication of prognosis.(2)It has important clinical reference value for detecting the early recurrence or metastasis to monitor serum p53 antibodies level on postoperative patients with ESCC. Key words: Esophageal squamous cell carcinoma; P53 gene; Enzyme linked immunosorbent assay; P53 antibody in serum