Abstract
AimTo analyse the expression of lncRNA-ANRIL and other related factors in different human body fluids, explore the clinical significance of ANRIL and validate whether ANRIL is interrelated with the renin–angiotensin system and NF-κB signalling pathway.MethodsNinety-one patients were included in this cross-sectional study and were divided into the NDM group (20 patients), DM group (25 patients), NPDR group (21 patients) and PDR group (25 patients). Basic information and samples of serum, aqueous fluid and vitreous fluid were collected before vitrectomy or intravitreal injection. The transcription and levels of ANRIL and other related factors were detected by RT-PCR and ELISA. Statistical Package for Social Sciences software was used for statistical analysis.ResultsANRIL expression varied among different groups and body fluids. There was no difference in ANRIL expression between the NDM and DM groups, but the level of ANRIL was significantly lower in the DM group than in the NPDR and PDR group. In vitreous fluid, ANRIL expression was positively correlated with Ang II, p65 and VEGF expression in the PDR group. The expression of ANRIL in serum was not significantly correlated with age or the random blood sugar but was positively correlated with diabetic duration and HbAc1 level.ConclusionsLevels of lncRNA-ANRIL are higher in DR patient and correlated with the progression of DR that may be used as an indicator to predict the development of DR. The activation of the RAS and the NF-κB pathway may be closely related to the upregulation of ANRIL.Clinical trial number ChiCTR1800017500. Registry Chinese Clinical Trial Registry.
Highlights
In 2016, more than 400 million people suffered from diabetic mellitus (DM) worldwide, and it is estimated that by 2030, the number of diabetic patients will be as high as 1 billion worldwide
The expression of ANRIL in all three body fluids was highest in proliferative diabetic retinopathy (PDR), while NDM and DM groups had similar values and non-proliferative diabetic retinopathy (NPDR) group showing intermediate values
To indirectly verify the relationship between ANRIL and Diabetic retinopathy (DR), this study analysed the difference in the levels of ANRIL and its related factors among nondiabetic, diabetic, NPDR and PDR patients from three body fluid sources: human peripheral blood, aqueous humour and the vitreous body
Summary
In 2016, more than 400 million people suffered from diabetic mellitus (DM) worldwide, and it is estimated that by 2030, the number of diabetic patients will be as high as 1 billion worldwide. There are approximately 10.2 million diabetic adults aged 40 and over in the USA [1]. Diabetic retinopathy (DR), one of the diseases that seriously endanger human vision and even cause irreversible blindness in the twenty-first century, has become a serious public health problem that cannot be ignored. The approximate prevalence rates of retinopathy and vision-threatening retinopathy are 40.3% and 8.2%, respectively [1]. The pathogenesis of DR is very perplexing because the mechanism of DR is not completely clear.
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