The clinical significance of decreased expression of calcium binding protein 39 in prostate cancer

Abstract

Objective To explore the role and clinical significance of calcium binding protein 39 (CAB39) protein in prostate cancer (PCa).Methods Using the two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) to screen CAB39 protein in PCa and adjacent tissues,and mass spectrometry analysis verification,to analyze the differentially expressed degree of CAB39 protein in 24 cases of PCa and adjacent tissues by immunohistochemical (IHC) technology,the combination of CAB39 IHC score and clinicopathological parameters of PCa patients were analyzed.Results Using the method of IHC,we found that the expression of CAB39 protein in adjacent tissues was significantly higher than that in PCa tissues (70.8％ vs 33.3％,P ＜0.01).The expression of CAB39 protein in different age stages of PCa patients,serum PSA levels,Gleason score and TNM stage in tumor group had no significant difference(P ＞0.05).Conclusion CAB39 protein is down-regulated expression in PCa,it may be prostate tumor suppressor. Key words: Calcium binding protein 39; Prostate cancer; Two-dimensional fluorescence difference gel electrophoresis