The clinical and histopathological study, the effects of platelet-rich fibrin on skin pedicle graft healing

Abstract

Background: Tissue engineering aims to repair or replace damaged tissue in response to various injury circumstances. One of these sectors is platelet-rich fibrin (PRF), extensively employed for soft tissue regeneration in recent years. Objective: The research aimed to examine the clinical and histological effects of allogenic PRF on skin pedicle grafts used to treat experimental avulsion wounds. Materials and method: Fifteen adult female goats were used for this study. Under heavy sedation and complete aseptic conditions, 2 skin marks (2×2 cm) were created on both sides of the trunk region of the animal. Two total thicknesses of the blotchy skin are cut carefully. Advancement (sliding) flaps (2×6 cm) are harvested parallel to the lines of most minor tension and slid over the adjacent wound. These wounds are allocated into two groups, the first one as the control group and the second group treated with autogenic platelet-rich fibrin. Each goat has two groups represented. The edges of the wounds were closed by 2-0 nylon monofilament sutures with a simple interrupted pattern. Results: Clinical examination showed the amount of wound exudation in the treated group significantly increased compared to the control group.