Abstract
Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity to both enzymes, respectively. The genes conferred resistance to β-lactams once expressed in Escherichia coliblaBKC-1 likely evolved from a putative ancestral Shinella gene with higher homology through duplication of a gene fragment.
Highlights
The high potential of Gram-negative bacteria to acquire exogenous DNA through horizontal gene transfer has allowed clinically relevant bacteria to acquire resistance toward many antibiotics [1, 2]
The genes code for weak class A carbapenemases sporadically identified in Klebsiella pneumoniae and P. aeruginosa isolates, respectively
All bacterial genomes and plasmids (n ϭ 610,187, downloaded March 2020) available in GenBank were searched for the blaGPC-1 and blaBKC-1-like genes, using DIAMOND v0.9.24.125 at a 70% identity cutoff [5]
Summary
The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella. G. Joakim Larssona,b aCentre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden bDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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