Abstract
Head and neck squamous cell carcinoma (HNSCC) refers to an epithelial malignant tumor that originates in the head and neck, and over 600,000 new cases are reported every year, However, the overall prognosis is still poor due to local recurrence and distant metastasis after surgery. The circ_0032822 has been reported upregulated in human oral squamous cell carcinoma; however, the detailed function or mechanism remains unknown. In this study, we confirmed the upregulation of circ_0032822 in HNSCC tumor tissues. Functionally, the overexpression of circ_0032822 significantly promoted the proliferation of HNSCC cell lines along with the S phase arrest and reduced apoptosis, while downregulation of circ_0032822 has the opposite effect in vitro. Mechanistic analysis showed that circ_0032822 acted as a competing endogenous RNA of miR-141 to diminish the repressive effect of miR-141 on its target E2F3. In conclusion, we demonstrated that circ_0032822 functions as a tumor oncogene in HNSCC and that its function is regulated via the miR-141/E2F3 axis.
Highlights
Head and neck squamous cell carcinoma (HNSCC) refers to an epithelial malignant tumor that originates in the head and neck and over 600,000 new cases are reported every year [1, 2]
The diagnosis and treatment have advanced in recent years, HNSCC still has a high incidence and mortality rate in developing countries [14, 15]
Identifying new molecular targets is a key step in improving the diagnosis, prognosis, and treatment of HNSCC
Summary
Head and neck squamous cell carcinoma (HNSCC) refers to an epithelial malignant tumor that originates in the head and neck and over 600,000 new cases are reported every year [1, 2]. Because of the closed-loop structure, circRNAs are not degraded by the exonuclease RNase R and show greater stability than linear RNAs [7]. Based on these features, circRNAs are defined as abundant, stable, and conserved molecules and usually exhibit tissue or developmental stagespecific expression [8]. MiRNAs regulate physiological and pathological processes by blocking protein translation or inducing mRNA degradation to inhibit target gene expression and are widely involved in many biological processes, such as cell metabolism, proliferation, differentiation and apoptosis [8, 9]
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