Abstract
Chromosomes of 13 enterogonid ascidians from the Pacific coasts of Japan were studied. The haploid number 20 was reported for Aplidium yamazii, A. multiplicatum, A. pliciferum and A. sagamiense; n=15 for Diplosoma mitsukurii; n=14 for Ciona intestinalis and C. savignyi; n=17 for Perophora japonica; n=11 for Ascidia sydneiensis samea and A. sydneiensis divisa; n=9 for Ascidia ahodori and A. rhabdophora; and n=8 for A. zara. The unique chromosome number found in A. sydneiensis among Ascidiidae was discussed briefly, in relation to the peculiar mantle musculature in this species. A new technique was employed for chromosome preparations of the ascidians, which allows one to examine the tissues kept in the Carnoy fixative solution 3:1 followed by 50% acetic orcein for a month.
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