Abstract
The karyotype of Nicotiana plumbaginifolia (2n=20) was determined by the analysis of 25 metaphase plates of both haploid and diploid plantlets. The material originated from root tip meristems and leaf protoplasts. Chromosome length ranged from 1.73 to 4.34 μm and the total length of the genome was 30.2 μm. The following chromosome pairs have been recognized: three metacentrics, two submetacentrics, one subtelocentric, one subtelocentric with satellites and three telocentrics.
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